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Transthyretin slowly exchanges subunits under physiological conditions: A convenient chromatographic method to study subunit exchange in oligomeric proteins

机译：运甲状腺素蛋白在生理条件下缓慢地交换亚基：一种方便的色谱方法，用于研究寡聚蛋白中的亚基交换

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Transthyretin (TTR) subunits were labeled with a charge-modifying tag to evaluate the possibility of subunit exchange between tetramers under physiological conditions. Starting with a mixture of two TTR homotetramers, one having all subunits tagged at the N termini and the other composed of untagged subunits, heterotetramer formation as a function of time and temperature was evaluated using ion exchange chromatography. The data indicate that the subunit exchange can occur under native conditions at physiological pH in vitro, albeit slowly. Wild-type TTR exchanges subunits on a timescale of days at 37°C and on a timescale of hours at 4°C. The familial amyloid polyneuropathy-associated variant V30M exchanges subunits at the same rate as wild-type TTR at 4°C but slower and less efficiently at 37°C. Small molecule tetramer stabilizers abolish TTR subunit exchange, supporting a dissociative mechanism.

机译：运甲状腺素蛋白（TTR）亚基用电荷修饰标签标记，以评估在生理条件下四聚体之间亚基交换的可能性。从两种TTR同型四聚体的混合物开始，其中一个在N端标记了所有亚基，另一个由未标记的亚基组成，使用离子交换色谱法评估了异四聚体的形成与时间和温度的关系。数据表明亚单位交换可以在自然条件下在体外生理pH下发生，尽管速度很慢。野生型TTR在37°C的几天的时间尺度上和4°C的小时的时间尺度上交换亚基。家族性淀粉样蛋白多神经病相关变体V30M在4°C时以与野生型TTR相同的速率交换亚基，但在37°C时变慢且效率较低。小分子四聚体稳定剂消除了TTR亚基的交换，支持了解离机制。

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Schneider, Frank; Hammarström, Per; Kelly, Jeffery W.;
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年度 2001
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1. Transthyretin slowly exchanges subunits under physiological conditions: A convenient chromatographic method to study subunit exchange in oligomeric proteins. [J] . Schneider F, Hammarstrom P, Kelly JW Protein Science: A Publication of the Protein Society . 2001,第8期

机译：运甲状腺素蛋白在生理条件下缓慢地交换亚基：一种方便的色谱方法，用于研究寡聚蛋白中的亚基交换。
2. Kinetic stabilization of an oligomeric protein under physiological conditions demonstrated by a lack of subunit exchange: Implications for transthyretin amyloidosis [J] . Wiseman RL, Green NS, Kelly JW Biochemistry . 2005,第25期

机译：缺乏亚基交换证明在生理条件下寡聚蛋白的动力学稳定：对运甲状腺素蛋白淀粉样变性的影响
3. Exchangeability of the b subunit of the Cl?-translocating ATPase of Acetabularia acetabulum with the β subunit of E. coli F1-ATPase: Construction of the chimeric β subunits and complementation studies [J] . Mikiko Ikeda, Hiromi Kadowaki, Hiromi Ikeda, Biochimica et biophysica acta. Bioenergetics . 1997,第1期

机译：Acetabularia acetabulum的Clα-易位ATPase的b亚基与大肠杆菌F1-ATPase的β亚基的可交换性：嵌合β亚基的构建和互补研究
4. Probing the interface between the sulfite reductase subunits, hemoprotein and flavoprotein, by H/D Exchange monitored by FT-ICR MS [C] . Yeqing Tao, Isabel Askenasy, Nicolas L. Young, ASMS Conference on Mass Spectrometry and Allied Topics . 2015

机译：通过FT-ICR MS监测的H / D Exchange探测亚硫酸盐还原酶亚基，血红蛋白和黄酮蛋白之间的界面
5. Subunit Exchange in Spinach Short-Form Rubisco Activase [D] . Forbrook, Dayna S. 2017

机译：菠菜短型Rubisco活化酶中的亚基交换
6. Transthyretin slowly exchanges subunits under physiological conditions: A convenient chromatographic method to study subunit exchange in oligomeric proteins [O] . Frank Schneider, Per Hammarström, Jeffery W. Kelly 2001

机译：运甲状腺素蛋白在生理条件下缓慢地交换亚基：研究寡聚蛋白中亚基交换的简便色谱方法
7. Kinetic Stabilization of an Oligomeric Protein under Physiological Conditions Demonstrated by a Lack of Subunit Exchange: Implications for Transthyretin Amyloidosis [O] . -1

机译：通过缺乏亚基交换证明了生理条件下的低聚蛋白的动力学稳定：对Transthyretin淀粉样蛋白病的影响

Transthyretin slowly exchanges subunits under physiological conditions: A convenient chromatographic method to study subunit exchange in oligomeric proteins

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